Research on the relation between hormones and unethical behaviors and tendencies has provided mixed results, hindering the understanding of the potential biological regulation of unethical behaviors and tendencies. We conducted an exploratory, longitudinal study (N = 257 women) allowing to estimate relations between, on the one hand, steroid hormones (testosterone, cortisol, estradiol, and progesterone) and conception probability and, on the other hand, a broad variety of measures related to unethicality (self-reported personality variables, cheating in committed relationships, self-serving economic dishonesty in a behavioral task, namely, the mind game). Contrary to theoretical assumptions of and results from some previous studies, we find no consistent relation between hormones and unethical behavior or tendencies in the majority of analyses. Yet, some small, exploratory associations emerged that call for (preregistered) replications, before more firm conclusions can be made.